TCR-seq / BCR-seq

Immune Repertoire Sequencing

TCR-seq & BCR-seq

T lymphocytes and B lymphocytes serve as pivotal mediators in antigen - specific immune responses. T lymphocytes orchestrate cell - mediated immune reactions and modulate immune functions, whereas B lymphocytes predominantly mediate humoral immunity via antibody synthesis. The antigen recognition process of these lymphocytes is contingent upon their respective surface receptors, namely the T cell receptor (TCR) and the B cell receptor (BCR). TCRs are heterodimers constituted by α and β polypeptide chains, while BCRs are composed of two heavy chains and two light chains. Structurally, both receptors are organized into V (variable), D (diversity), J (joining), and C (constant) regions. Notably, the TCR α chain and BCR light chains lack the D segment. Through V(D)J recombination and rearrangement, TCRs and BCRs attain significant diversity, facilitating the recognition of a wide array of antigens.

Workflow

Bioinformatic Analysis

Basic Analysis

1. Raw data quality control and filtering 2. Genome alignment 3. Clonotype assembly 4. Basic clonotype analysis metrics 5. CDR3 nucleotide & amino acid length distribution 6. CDR3 clustering analysis 7. V gene usage 8. J gene usage 9. VJ gene usage 10. CDR3 clonotype length distribution 11. CDR3 amino acid motifs 12. V gene-specific CDR3 length distribution 13. Immune polymorphism analysis

Group Analysis

1. Inter-group CDR3 length difference 2. Inter-group V gene usage difference 3. Inter-group J gene usage difference 4. Inter-group VJ gene usage difference 5. Inter-group diversity metrics difference

Advanced Analysis

1. Clonotype diversity 2. Public clonotypes 3. Immune repertoire similarity

Demo

Sample Requirements

cells, tissues, blood, bone marrow, and others

For detailed sample type specifications and quantity requirements, please contact us directly.